IRN AP09058158 Study of export-oriented bioresource — Artemia franciscana for biological justification development on their introduction, to increase productivity of RK saline waterbodies
This work will provide an opportunity to increase the productivity of bitter-salt water reservoirs of the country by implementation a new biological species for Kazakhstan – Artemia franciscana. The novelty of the work lies in the fact that there has been no work aimed at increasing the stocks and quality of Artemia’s cysts so far this year in Kazakhstan.
To study opportunities, the main criteria of crustacean Artemia franciscana survival and to compare production characteristics of two populations of valuable bioresource – Artemia parthenogenetica, Artemia franciscana, for the purpose of increase in productivity bitter-salty RК reservoirs, for receiving high-quality competitive products for implementation in the internal and external markets.
The implementation of the tasks will help to reveal the interpopulation competitiveness of two populations of crustaceans, the degree of their survival, as a consequence, which will determine the prevalence of more productive populations. There are practically no studies that determine the economic aspects of the cultivation of Artemia in the regions of the Republic of Kazakhstan, and therefore, there is a need to conduct work to assess the influence of various factors on the productional population characteristics of Artemia franciscana during the introduction of Artemia cysts into natural water bodies.
The physical-geographical and morphological characteristics of bitter-salty water reservoirs of Pavlodar region were examined using navigation devices, unmanned aerial vehicles, etc. Studies were carried out in bitter-salty water reservoirs to determine the concentration of Artemia cysts. The physical-geographical and morphological characteristics of bitter-salty water reservoirs of Pavlodar region were examined using navigation devices, unmanned aerial vehicles, etc. Studies were carried out in bitter-salty water reservoirs to determine the concentration of Artemia cysts. Research work was carried out on selected salt reservoirs (Pavlodar region). An assessment of the hydrochemical regime of selected reservoirs of the Republic of Kazakhstan was carried out, and the structure of the Artemia population of the studied reservoirs was determined. Materials on hydrobiology, hydrochemistry, genetics and biotechnology have been collected. Research work was carried out on selected bitter-salty water reservoirs of the Republic of Kazakhstan. Collected data on habitat conditions, distribution and population structure of Artemia in water reservoirs. The genetic diversity of cysts and crustaceans of Artemia parthenogenetica and Artemia franciscana was assessed by molecular genetic methods (nuclear and mitochondrial D1IK markers). The received material was processed and the data was analyzed, 1 (one) article was published in a reviewed foreign publication recommended by the CQASES MES RK. The analysis of the received data was carried out, an interim report on research was drawn up and submitted in accordance with GOST.
The necessary equipment has been made. Work has been carried out to identify the effectiveness of the use of various feed components in the cultivation of juvenile of Artemia. Studies have been carried out to determine suitable food objects for Artemia larvae and nauplii.The biochemical composition and energy value of the biomasses of two crustacean populations and their cysts were assessed for commercial use. The laboratory determined the biochemical composition of Artemia cysts, followed by analysis of the results.Research work was carried out on selected bitter-salty water reservoirs (Aral Sea: Tushchybas and Chernyshev bays). An assessment was made of the hydrochemical regime of selected reservoirs of the Republic of Kazakhstan, and the structures of the Artemia population in the studied bays were determined. Materials on hydrobiology, hydrochemistry, genetics and biotechnology were collected.The optimal stocking density of larvae and juvenile invertebrates under various conditions was established. Experimental work has been carried out.Research work was carried out on selected bitter-salty water reservoirs of the Republic of Kazakhstan. Data on habitat conditions, distribution and structure of the population were collected, 2 (two) articles were published in foreign and (or) domestic publications recommended by the CQASSHE. 1 patent was obtained for a utility model for transporting or catching Artemia cysts. 1 training seminar was held.The genetic diversity of cysts and crustaceans of Artemia parthenogenetica and Artemia franciscana was assessed by molecular genetic methods (nuclear and mitochondrial DNA markers). Differences between populations of the crustacean Artemia parthenogenetica and Artemia franciscana were determined using microsatellite (STR) and mitochondrial (CtB gene) DNA markers in the Tushchyba Bay of the Aral Sea. An analysis of the data obtained was carried out and a report was prepared in accordance with GOST.
Work has begun on the development of temporary standards for the conditions of keeping and growing two populations of Artemia crustaceans (hydrochemical regime, biological data, calculation of the optimal stocking density) Franciscan. To determine the size and growth rate of crustaceans, morphometric measurements (body length and abdominal width) of Artemia were performed. Research work was carried out on selected bitter-salty water bodies (North-Kazakhstan region, near the city of Petropavlovsk). An assessment of the hydrochemical regime of selected reservoirs of the Republic of Kazakhstan was carried out, the structure of the Artemia population of the studied reservoirs will be determined. The hydrological and hydrochemical parameters of the habitat of the crustacean, their seasonal variability in 4 lakes (Lake Zhamantuz, Lake Mengesor, Lake Stanovoe, Lake Kalibek) were established in the early summer period of 2023. Phytoplankton was studied as a food base for Artemia crustaceans. The morpho-biological and population characteristics of two Artemia populations were revealed. Studies of Artemia crustacean populations were carried out to determine the places of their concentration. The sensitivity of two types of Artemia to fluctuations in the parameters (salinity, oxygen content, temperature and pH value) of the environment is being assessed. An assessment of the genetic polymorphism of cysts, larvae and nauplii of Artemia crustaceans of two species was carried out, a molecular genetic analysis of various populations of the crustacean Artemia parthenogenetica was given. As a result of sequencing of the gene for cytochrome oxidase (COI) and cytochrome b (ctb) of mitochondrial DNA, a fragment of the nucleotide sequence with a length of 648 and 750 base pairs was obtained. A seminar was organized to disseminate knowledge among nature users and fish farm workers to attract public attention on the topic “Modern methods for studying the biological characteristics of Artemia gill crustaceans and assessing the quality of cysts.” The development of a biological justification for increasing the productivity of saline and/or hypersaline water bodies of the Republic of Kazakhstan has begun. 1 scientific article has been published in a domestic journal recommended by the CQAFSHE.